Researcher Database

（Last updated : 2023-02-08 12:02:11）

NEGORO RYOSUKE

Department / Course College of Pharmaceutical Sciences Department of Pharmacy

Title / Position Assistant Professor

Academic background
1.	2015/04～2019/03	Doctorial Course │ Graduate School of Pharmaceutical Sciences │ Osaka University │ Completed Doctor of Pharmacy
2.	2009/04～2015/03	Faculty of Pharmaceutical Sciences │ Setsunan University. │ Graduated

Business career
1.	2019/04/01 ～ 2024/03/31	│ Laboratory of Molecular Pharmacokinetics, Collegeof Pharmaceutical Sciences , │ Ritsumeikan University

Research activities
1.		The International Society for Stem Cell Research
2.		The Pharmaceutical Society of Japan
3.		The Japanese Society for the Study of Xenobiotics

Subject of research
1.	Prediction of intestinal drug absorption and intestinal drug metabolism

Research summary
Model construction of drug intestinal absorption and drug intestinal first-pass metabolism Administered drug are absorbed and metabolized in the small intestine. A model that could evaluate drug absorption and metabolism in the small intestine would be useful for pharmacokinetic study. Currently, experimental animals and cancer cell lines such as Caco-2 cells are widely used as intestinal models because primary human small intestinal epithelial cells are difficult to obtain and culture. However, these existing models do not accurately reflect the human small intestine functions. Recently, Molecular biology research methods such as genome editing technology and gene expression control technology using viral vectors are rapidly developing. In this study, we will work on constructing models that could predict drug intestinal absorption and drug metabolism using by molecular biological technologies such as genome editing technology and viral vectors.

Present specialized field
Biological pharmacy, Medical pharmacy, General anatomy (including histology/embryology) (Keyword：Intestinal drug absorption, Intestinal drug metabolism, drug transporter, drug-metabolizing enzyme, iPS cells)

Papers
1.	2023/03	Elucidation of the liver pathophysiology of COVID-19 patients using liver-on-a-chips. │ PNAS Nexus │ 2 (3),pp.029 (Co-authored)
2.	2023/02	Generation of Caco-2 cells with predictable metabolism by CYP3A4, UGT1A1 and CES using the PITCh system. │ Drug Metab. Pharmacokinet. │ In Press (Co-authored)
3.	2022/02	Generation of Caco-2 cells stably expressing CYP3A4·POR·UGT1A1 and CYP3A4·POR·UGT1A16* using a PITCh system. │ Arch. Toxicol. │ 96 (2),pp.499-510 (Co-authored)
4.	2021/08	Usability of Polydimethylsiloxane-Based Microfluidic Devices in Pharmaceutical Research Using Human Hepatocytes. │ ACS. Biomater. Sci. Eng. │ 7 (8),pp.3648-3657 (Co-authored)
5.	2021/04	Asymmetric profiles of infection and innate immunological responses in human iPS cell-derived small intestinal epithelial-like cell monolayers following infection with mammalian reovirus. │ Virus Res. │ 296,pp.198334 (Co-authored)
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Academic conference presentation
1.	2019/12/11	Development of human iPS cell-derived intestinal epithelial cells for drug metabolism and absorption test (日本薬物動態学会第34回年会)
2.	2019/12/11	Establishment of intestinal and hepatic drug transporter-knockout human induced pluripotent stem cell lines for pharmacokinetic studies (日本薬物動態学会第34回年会)
3.	2019/12/10	Maturation of the enterocyte-like cells differentiated from human iPS cells using Vinblastine (日本薬物動態学会第34回年会)
4.	2019/07/30	GENERATION OF HIGH-PURITY HUMAN IPS CELL-DERIVED INTESTINAL EPITHELIAL CELLS FOR PHARMACEUTICAL RESEARCH (12th International ISSX Meeting)
5.	2018/10/28	Asymmetric activation of innate immunity after apical and basolateral infection of polarized human intestinal epithelial cells with reovirus (第66回日本ウイルス学会学術集会)
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External Researcher ID
ORCID ID
0000-0002-6567-575X
Researcher ID