Researcher Database

（Last updated : 2023-03-15 11:52:30）

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NISHIZAWA Mikio

Department / Course College of Life Sciences Department of Biomedical Sciences

Title / Position Professor

Other Affiliations
1.	Graduate School of Life Sciences
2.	Ritsumeikan Asia-Japan Research Organization Asia-Japan Research Institute

Academic background
1.	1987/09 Degree Acquisition	│ Tohoku University Ph.D. (Doctor of Medicine)
2.	～1987/03	Doctorial Course │ School of Medicine, Graduate Course │ Tohoku University │ Completed Medical biochemistry Ph.D.
3.	1983/05 Degree Acquisition	│ Ministry of Health, Labour and Welfare, Japan The Physician's License from the Ministry of Health, Labour and Welfare, Japan
4.	～1983/03	Department of Medicine │ School of Medicine │ Toyama Medical and Pharmaceutical University │ Graduated

Business career
1.	2008/04/01	Professor │ Institute of Science and Engineering, College of Life Sciences, │
2.	2007/04/01 ～ 2008/03/31	Professor │ Department of Bioscience and Biotechnology, College of Science and Engineering, │
3.	1997/04/01 ～ 2007/03/31	Lecturer │ the Department of Medical Chemistry │ Kansai Medical University
4.	1995/05/01 ～ 1997/03/31	Assistant researcher │ the Department of Medical Chemistry │ Kansai Medical University
5.	1993/06/01 ～ 1995/04/30	Post-doctoral fellow │ Department de Biologie Moleculaire et Biochimie, Faculte des Sciences, │
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Research activities
1.		The Japanese Biochemical Society
2.		The Japanese Biochemical Society, Kinki Branch
3.		The Molecular Biology Society of Japan
4.		The Japanese Cancer Association
5.		The Japanese Society for Research of Hepatic Cells
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Subject of research
1.	(1)Regulation mechanism of cytokine gene expression by natural antisense transcripts and application to development of novel drugs

Research summary
Regulation mechanism of cytokine gene expression by natural antisense transcripts and application to development of novel drugs Central dogma, namely, DNA makes RNA makes protein, indicates that information encoded in DNA (blueprint of life) is transmitted to messenger RNA (mRNA) and then translated to protein that works in the cell. Recently it is reported that there are number of RNA that is not translated to protein in mammalian cells. The untranslated RNA (non-coding RNA) was thought to have no useful functions. We have found antisense transcripts (antisense RNA), one type of non-coding RNAs, and elucidated that the antisense RNA stabilizes mRNA and regulates gene expression. The antisense RNA is transcribed from a gene encoding inducible nitric oxide synthase (iNOS). This enzyme catalyzed the reaction to produce nitric oxide (NO), which kills viruses and bacteria during infection, while excess NO may cause tissue damage or septic shock. We attempt to degrade mRNA by inhibiting stabilizing activity of the antisense RNA finally to reduce excess NO production. Collaborators: Ritsumeikan University: Tominori Kimura, Tadayoshi Okumura; Yukinobu Ikeya (Daiichi University of Pharmacy), Masaki Kaibori (Kansai Medical University), Koji Wakame (Hokkaido Pharmaceutical University School of Pharmacy), Kenji Sato (Kyoto University).

Present specialized field
Pathological Medical Chemistry, Medical biochemistry, Pharmacology (Keyword：Natural antisense transcript, cytokine, chemokine, nitric oxide, inducible nitric oxide synthase, gene, gene regulation, hepatocytes, inflammation, drug)

Books
1.	2022/10/19	The Natural Antisense Transcript-Targeted Regulation Technology Using Sense Oligonucleotides and Its Application │ ,pp.1-19 (Co-authored)
2.	2016/10	Clinician's Guide to AHCC: Evidence-Based Nutritional Immunotherapy │ ,pp.109-115,134-143 (Co-authored)

Papers
1.	2022/08	Anti-inflammatory effects of hydrophobic constituents in the extract of the root cortex of Paeonia suffruticosa │ Bioactive Compounds in Health and Disease │ 5 (8),pp.160-173 (Co-authored)
2.	2022/06	Anti-inflammatory effects of anthraquinones of Polygonum multiflorum roots │ Bioactive Compounds in Health and Disease │ 5 (6),pp.136-148 (Co-authored)
3.	2022/03	Bitter melon fruit extract enhances intracellular ATP production and insulin secretion from rat pancreatic β-cells │ British Journal of Nutrition │ 127 (3),pp.377-383 (Co-authored)
4.	2022/03	Omeprazole Increases Survival Through the Inhibition of Inflammatory Mediaters in Two Rat Sepsis Models. │ Shock │ 57 (3),pp.444-456 (Co-authored)
5.	2022/02	Detection of Decarboxylated Amino Acids after in Vitro Protease Digestion of the Hydrophilic Fraction of Crude Drug Extracts │ Biological and Pharmaceutical Bulletin │ 45 (2),pp.169-177 (Co-authored)
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Academic conference presentation
1.	2023/03/04	Anti-inflammatory Effects of Traditional Japanese Kampo Medicine (Malang International Conference on Herbs and Toxicants (MIC-HT))
2.	2022/12/02	Improvement of insulin sensitivity and inhibition of hepatic gluconeogenesis by components in bitter melon fruits
3.	2022/12/02	Identification of components of Zenko that suppress nitric oxide production in primary cultured hepatocytes (MBSJ2022)
4.	2022/12/02	White-skinned sweet potato (Ipomoea batatas L.) acutely suppresses postprandial blood glucose elevation by improving insulin sensitivity
5.	2022/10/21	Crude Drugs of Traditional Japanese Kampo Medicine (The 1st International Conference on Medicinal Plants: The 62nd Meeting of Working Group on Indonesian Medicinal Plant)
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Works
1.	2022/07/13	Traditional Japanese Medicine and Functional Foods (IPB（オンライン開催）)
2.	2022/04/06	Traditional Japanese Medicine and Crude Drugs (Vietnam National University of Agriculture)

Winning science prize
1.	1992/01	Alexander von Humboldt Foundation Alexander von Humboldt-Forschungsstipendium (1992)-

Grants-in-Aid for Scientific Research -KAKENHI-
1.	2015/04 ～ 2018/03	Development of next-generation nucleic acid medicines based on the anti-influenza viral regulatory antisense RNA │ Grant-in-Aid for Scientific Research (C)

Acquisition patent
1.	2014/03	COMPOUND, MEDICINE, ANTI-INFLAMMATORY, COSMETIC, FOOD AND DRINK, AND METHOD FOR PRODUCING COMPOUND （特願2013-181052）

Research exchange preferred theme
1.	Study on regulation mechanism of gene expression and application to design of drugs Study on regulation mechanism of gene expression (focused on the genes induced during inflammation, such as iNOS gene) and application to design of drugs (including anti-inflammatory drugs) Commisioned research Joint research

External Researcher ID
ORCID ID
0000-0002-1117-2807